Volume :
2
Issue :
4
Abstract :
The purpose of this study was to isolate and identify the endophytic fungi from the roots of Rhamnus prinioides (gesho) plant as well as to examine their antibacterial activity. All the root samples were subjected to a three step surface sterilization procedure. After proper sterilization of the surface, roots of Rhamnus prinioids were evenly placed in Petri dishes containing potato dextrose agar (PDA) medium. The Petri dishes were incubated at 30 °C and monitored every day to check the growth of microorganism colonies from the root segments. Total 15 root segments of Rhamnus prinioids plants were subjected for isolation of endophytic fungi. Out of which 9 fungal strains were isolated and identified as Alternaria Sp. (N=3), Aspergillus Sp. (N=5) and Curvularia Sp. (N=1). Among 9 isolates of endophytes only 6 isolates were effective against selected bacteria. Among 6 endophytes 50% showed to effectiveness E. coli and P. aeruginosa. Whereas, 66.66% isolates were effective against S. aureus. Two isolates of Alternaria Sp. and 3 isolates of Aspergillus Sp. were effective against all selected bacteria. Whereas, 1 isolate of Curvularia Sp. was effective against only S. aureus. This study addressed the scientific knowledge gap and explored whether Ethiopian plant endophytes, may be a potential natural resource to yield useful biologically active compounds.