Volume :
3
Issue :
1
Abstract :
A simple, precise, accurate, economical and reproducible HPLC method for estimation of atorvastatin in tablet dosage form has been developed. Quantitative HPLC was performed with SHIMADZU LC2010c HT with class-10vp Software with UV-Visible Detector (SPD-IOA), PUMP (LC-IOAT) and (LC-IOATvp). Waters C8, 5µm, 25cmx4.6mm i.d. column was used in the study. The mobile phase of ACN: phosphate buffer (pH 4) = 6:4, diluents of water: ACN (50: 50) were used for sample preparation in this study. The final pH of mobile phase was 4. The conditions optimized were: flow rate (1 ml/minute), wavelength (246 nm) and run time was 25 min, column temperature was maintained at 500C. Retention time was found to be 6.975 min. The linearity was found to be in the concentration range of 10-100 mg/ml. The developed method was evaluated in the assay of commercially available tablets ATORVA-20, 40 containing atorvastatin respectively. The amount of drug in tablet was found to be 20.36, 40.2 mg/tab for the brands. Results of analysis were validated statistically and by recovery studies. The recovery studies 99.67 % was indicative of the accuracy of proposed method. The precision was calculated as repeatability, inter and intraday variation (%RSD) for the drug. By using the method, stability of the drug has been studied.