Volume :
4
Issue :
1
Abstract :
The present study was aimed to investigate the pharmacognostical, phytochemical, in-vitro toxicity and in-vitro hepatoprotective activity of Bridelia tomentosa root. Pharmacognostical and phytochemical analysis were carried out as per standard procedures. In-vitro toxicity study was performed using MTT assay on BRL3A cell line. In-vitro hepatoprotective activity was performed using MTT assay on paracetamol treated BRL3A cell line. The phytochemical screening showed the presence of different phytoconstituents. Petroleum ether extracts showed the presence of phytosterols, gums and mucilage’s. Chloroform extracts showed the presence of glycosides and steroids. Methanol extract showed the presence of saponins, tannins, phenolic compounds and flavonoids. HPLC analysis showed the presence of various phytochemical. However, Gallic acid, Rutin and Quercetin were the major phytochemical. The CTC50 of Bridelia tomentosa root was greater than 1000 µg/ml. Hence, the powdered root of Bridelia tomentosa haven’t shown any significant toxicity towards BRL3A cell line. Bridelia tomentosa root has offered protection for BRL3A cell line against paracetamol at the dose of 500 µg/ml. Further, increase in concentration to 1000 µg/ml has not offered protection. The study concludes that the Bridelia tomentosa roots is safe and offers significant hepatoprotection against paracetamol. However, this pilot study data require further validation using molecular level studies and clinical trials.