Volume :
2
Issue :
3
Abstract :
Two simple, rapid, precise and economical spectrophotometric methods have been developed for simultaneous estimation of sitagliptin phosphate and metformin hydrochloride from tablet dosage form. First method, Simultaneous equation method, involves the measurement of absorbances of sitagliptin and metformin at the wavelengths of 267nm (λmax of sitagliptin) and 232 nm (λmax of metformin). Second method involves second order derivative spectroscopy using 249 nm and 278 nm as zero crossing points for sitagliptin and metformin respectively. For the spectrophotometric method water was used as solvent .Linearity was observed in the concentration range of 5-50µg/ml for both sitagliptin and metformin for both the methods. The accuracy and precision of the methods were determined and validated statistically. Both the methods showed good reproducibility and recovery with % RSD less than 2. The proposed methods were found to be rapid, specific, precise, accurate and cost effective quality control tool for the routine analysis of sitagliptin and metformin in bulk and combined dosage form.