Volume :
1
Issue :
4
Abstract :
The present study was carried out to evaluate the in vitro cytotoxic activity of unexploited plants, heartwood of Caesalpinia sappan Linn and roots of Annona squamosa Linn. on A-549 lung cancer cell line, which are indigenous to India. Different concentrations of the methanolic extracts of heartwood and root parts of the plant (1000, 500, 250, 125, 50, 25, 12.5 µg/ml) were subjected to cytotoxic study against A-549 lung cancer cell lines by Trypan blue dye exclusion technique. In addition, a phytochemical screening of the ethanolic extracts was done. The phytochemical screening demonstrated the presence of different types of compounds like flavonoids, triterpenoids, alkaloids, acetogenins, phenols and sterols. The maximum reducing power of the Caesalpinia sappan and Annona squamosa extract at 680nm was found to be 0.976 ± 0.051 at 1000 µg/ml and 0.953 ± 0.037 at 1000 µg/ml respectively. The inhibition percentage with regard to cytotoxicity was found to be 87 % at 1000 µg/ml with IC50 value of 49 ± 0.03 µg/ml for Caesalpinia sappan and 85 % at 1000 µg/ml with IC50 value of 47 ± 0.02 µg/ml for Annona squamosa respectively. The ethanolic extracts of Caesalpinia sappan and Annona squamosa are showing potent cytotoxic activity against A-459 lung cancer cell line.