Volume :
1
Issue :
2
Abstract :
Antibiotics are the secondary metabolites produced by microorganisms. Most of the Antibiotics are produced by Bacteria, Fungi, and Moulds etc. Out of which 2/3rd are produced by Actinomycetes. Actinomycetes are Gram + ve filamentous Bacteria and are mostly present in soil, hence soil screening is advantageous to isolate Actinomycetes. This work is designed to isolate antibiotic producing Actinomycetes from soil and to screen the productivity of antimicrobial substances of the isolated Actinomycetes. Due to resistance produced by micro-organism against all commonly used antimicrobial agents, there is a need of newer antibiotics. Only 1% percent of the microorganisms in soils are cultured. In order to explore the hidden diversity, soil screening of antimicrobial agents are performed for the detection of non culturable microorganism. Four soil samples have been collected. By crowded plate technique Actinomycetes were isolated. Anti-microbial activity of the isolates was performed by spektra plak technique. Fermentation was carried out for the isolate which have shown broad spectrum activity. The anti-microbial compounds were isolated by solvent extraction method by using ethyl acetate and the compounds were partially purified by evaporation. The anti-microbial activity was studied for the compounds which have been isolated by agar well method. 62.79 % of Actinomycetes which were isolated from the soil had shown antibacterial activity, 2.32 % had shown anti-fungal activity and 16.28 % had shown broad spectrum activity. These studies were done in partially purified antimicrobial compounds. It will be worthwhile to conduct this study with the purified form of antimicrobial compounds.