Volume :
1
Issue :
2
Abstract :
A validated HPLC method for simultaneous estimation of Zinc carnosine and Aceclofenac in pharmaceutical dosage forms. Chromatography was carried out on a C18 column [250mm, 4.6m, 5µm] using a mixture of potassium di-hydrogen phosphate buffer: Acetonitrile: methanol (50:30:20 v/v) as the mobile phase at a flow rate of 1 ml/min. Detection was carried out by using PDA detector. The retention time of the drug was 2.258 and 6.690 min. The method produced linear responses in the concentration range of 2 to 10μg/ml for both drugs. The LOD and LOQ values were found to be 33.4, 101.3 and 2.473, 7.495 ng/ml respectively. The method was validated for linearity, precision, accuracy, LOD &LOQ in accordance with ICH guidelines. The proposed method was found to be applicable for determination of the drug in tablet dosage forms.