Volume :
5
Issue :
2
Abstract :
New Spectrophotometric Q-Absorbance Ratio method has been developed for the simultaneous estimation of Arterolane maleate and Piperaquine phosphate in tablet dosage form. UV spectrophotometric method, methanol was used as a solvent. Objective To develop simple, accurate, linearity, precision and reproducible UV Spectroscopic method for Arterolane maleate and Piperaquine phosphate in routine analysis. Method Aliquots of stock solution were further diluted with methanol to get working solution of 2.5-8.75μg/ml for Arterolane maleate and 12.5-43.5 μg/ml for Piperaquine phosphate working standards were scanned between 200 - 400 nm which shows the maximum absorbance at 276nm. Results The Iso -absorptive point was found to be 242 nm. Calibration curve were linear over a concentration range of 2.5-8.75 μg/ml for Arterolane maleate and 12.5- 43.5 μg/ml for Piperaquine phosphate. Accuracy of method was determined through recovery studies which were found 99.36%-101.22% for Arterolane maleate and 99.46%- 100.80% for Piperaquine phosphate. Method was found to be reproducible with relative standard deviation for intra- day and inter-day precision to be < 1.5%. Conclusion This method was found to be simple, Accurate, precise and reproducible. The proposed UV method can be applicable for the simultaneous estimation of both the drugs in tablet dosage form.