Abstract :
Background: Silver nanoparticles (AgNPs) are an integral part of nanotechnology and setting up new trends in pharmaceutical field due to its wide therapeutic applications. Aim: In the present research, green synthesis of AgNPs using leaf extracts of Phyllanthus fraternus with an evaluation of their cytotoxicity activity against hepatic and breast cancer cell lines. Materials and Methods: AgNPs were used to characterized by ultraviolet (UV)-visible spectrophotometry, Fourier-transform infrared (FTIR) spectrophotometry, scanning electron microscopy (SEM) with elemental mapping, transmission electron microscope (TEM), and X-ray diffraction (XRD). In vitro cytotoxicity studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay method. Results and Discussion: AgNPs were identified by the change of color and their absorption at 420 nm measured by UV-visible spectroscopy, FTIR spectral analysis confirmed phenolic compounds presence, morphology and size visualized in SEM, and TEM used for the determination of size, shape, and light scattering analysis. Synthesized AgNPs were spherical in shape with size
Keyword :
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay hepatic cancer cell line 2, green, silver nanoparticles, breast cancer cell line, Phyllanthus fraternus