Abstract :
The present study examined the genetic variations of the newt (Triturus vittatus vittatus) (Urodela) from
different breeding sites on thesouthern border of its distribution using mitochondrial DNA of 12S and 16S, and
nuclear gene, rhodopsin. In an analysis of DNA sequences of rhodopsin (163 bp) from various breeding sites in
different habitats, no genetic variation among the different populations was found. Moreover, using molecular
variance (AMOVA) analysis no significant differences were detected. On the other hand, a great variance was found
in comparing the two mitochondrial genes, the 12S–rRNA (357 bp) fragment and the 16S–rRNA (521 bp) fragment
in regions of the different populations. A comparison between different populations using AMOVA showed great
dissimilarity regarding the breeding sites of newts on thesouthern border of their distribution and at the highest
altitudes.
Keyword :
Genetic variations, Mitochondrial DNA, Triturus