Abstract :
Objectives: The investigation of antimicrobial activity of water-alcohol plant extracts Ruta graveolens L., Nepeta cataria L., and Stellaria media (L.) Vill. and the detection of the synergism of their antimicrobial action with erythromycin against the skin isolates of Staphylococcus aureus with different mechanisms of acrolides, lincosamides, and streptogramins (MLS)-resistance. Materials and Methods: It has been carried out by the micromethod of diffusion into agar and the method of diffusion of active substances into agar using paper disks. Results: The direct antimicrobial effect of the investigated extracts to the test strains was established. The synergistic antimicrobial effect of 1/4 and 1/64 minimum decreasing concentration of erythromycin to all strains of staphylococcus was demonstrated by extracts from R. graveolens L. The biologically active substances of medicinal plants more effectively restore the sensitivity to erythromycin for staphylococcus with low MLS‑resistance through blocking reflex mechanisms. High-level MLS-resistance undergoes to a modification influenced considerably less. Conclusion: The investigated plant extracts of R. graveolens L. show antimicrobial activity against all tested strains of microorganisms: Gram-positive and Gram-negative bacteria, Candida tropicalis. N. cataria L. extract influenced some of the tested cultures of microorganisms. Extract of S. media (L.) Vill. inhibited the growth of microorganisms, mostly to streams of Streptococcus aureus ATCC 25923 and S. aureus ATCC 6538 in a dose of 200 mcg/ml.
Keyword :
Key words: Antimicrobial effect, erythromycin, plant extracts, synergism of antimicrobial action