Abstract :
Aim: Plants are ancient source of medicine due to the presence of various bioactive molecules present in different parts of the system. In this study, we investigated the biologically active extract and isolated one pure compound from the stem bark of Pisonia grandis R.Br. Materials and Methods: 1H and 13C nuclear magnetic resonance (NMR), mass spectroscopy, Fourier-transform infrared spectroscopy, thin-layer chromatography, potassium bromide, and DMSO-d6 were used. Results and Discussions: The pure compound was isolated and identified as β-sitosterol glucoside, and the structure was characterized by 1H and 13C NMR spectroscopy analysis. Later on with the structural elucidation with 13CNMR, the mass spectrum was also revealed, the m/z ratio of β-sitosterol glucoside was found to be 613[M+2H20+1]+. Conclusion: The study implies that though the compound isolated is abundantly known and is present in majority of the plants, the work held out is first to report for the presence of β-sitosterol glucoside from the ethyl acetate extract in the stem bark of P. grandis R.Br.
Keyword :
Key words: Column chromatography, isolation, Pisonia grandis, spectral analysis, β-sitosterol glucoside