Abstract :
Objective: This study was planned to determine the effect of certain vitamin applications on antioxidant and
oxidant parameters in the osteoblast cell line exposed to sodium fluoride in vitro and to evaluate the protective
role of certain vitamins against possible toxic effects of fluoride.
Materials and Methods: Cells were replicated in vitro conditions with regular passaging 2-3 times weekly. MTF
viability test was used to determine IC50 of NaF (5000?M) and proliferative doses of vitamins (Vitamin A: 10?M,
Vitamin D: 10?M, Vitamin E: 60?M, Vitamin C: 100?M) for hFOB 1.19 cells. Cells were sown in flasks as so
to be 106
. The study groups were identified as control, NaF, vitamins and NaF+vitamins. After incubation for 24
hours, cells treated with trypsin were prepared by freeze/thaw method and MTT viability test, TAS, SOD, GSH,
CAT, TOS and MDA analyzes were performed on these samples.
Results: In the hFOB 1.19 cell line, TAS levels decreased significantly in the NaF group (p?0.05), but were close
to the control group in NaF+vitamin groups with the exception of vitamin C. However, there was no difference
between the groups in terms of GSH level and CAT and SOD activities when the control and NaF groups were
compared. It was observed that TOS level increased significantly in the NaF group (p
Keyword :
NaF, cell culture, antioxidant, oxidative state, vitamins