Abstract :
Antifungal potential of ß citronellol was studied on C. albicans, a commensal that causes both superficial and systemic infection in immunocompromised humans. This non toxic natural compound displayed a minimum inhibitory concentration MIC of 200 µg mL and was fungicidal at 350 µg mL. On YPD agar, it formed inhibition zones ZOI of 16.2 mm, 18.15 mm and 19.25 mm respectively, at MIC, 2MIC and 3MIC while fluconazole FLC formed ZOI of 20.43 mm at its MIC disc diffusion assay . No growth was observed above 2MIC on solid media spot assay and = MIC in liquid YPD media. Hydrolytic enzyme secretion decreased in the presence of ß citronellol and was concentration dependent. At MIC, the reduction in phospholipase activity 54.08 was greater than the reduction in proteinase activity 40.6 . There was concentration dependent decrease in total ergo sterol content by 19 , 40 , 91 and 100 , respectively at MIC 8, MIC 4, MIC 2 and MIC values of ß citronellol. FLC at MIC showed an inhibition of only 64 . Biofilm formation reduced by 71.13 at MIC. ß citronellol, hence has immense antifungal potential and significantly inhibits growth, ergosterol levels, hydrolytic enzyme secretion, adhesion and biofilm formation in C. albicans at MIC and sub MIC values. Sumit Kumar Rastogi | Yamini Sharma | Nikhat Manzoor "Effect of Β-Citronellol on Hydrolytic Enzyme Secretion, Ergo sterol Biosynthesis and Bio film Formation in C. Albicans" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-2 | Issue-5 , August 2018, URL: https://www.ijtsrd.com/papers/ijtsrd18305.pdf Paper URL: http://www.ijtsrd.com/biological-science/microbiology/18305/effect-of-β-citronellol-on-hydrolytic-enzyme-secretion-ergo-sterol-biosynthesis-and-bio-film-formation-in-c-albicans/sumit-kumar-rastogi
Keyword :
Candida albicans; ß-citronellol; antifungal susceptibility; cytotoxicity; biofilms; membrane integrity